Introduction
ScanImage 2015 and later allows users to configure their system for resonant scanning or linear scanning (=galvo only scanning). A microscope with two laser paths can combine both methods to allow for simultaneous imaging and photostimulation.
The sections below describe the basic acquisition and control hardware requirements for both imaging systems.
- Resonant Scanning
- Linear Scanning (Galvometric scanning)
Resonant Scanning
ScanImage uses vDAQ for data acquisition and microscope control. vDAQ is an all-in-one data acquisition board that controls the most advanced laser scanning microscopes.
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The 2U rackmountable vDAQ breakout shows the main functionality of the data acquisition system:
- 4 fast analog inputs for acquiring PMT data
- 12 2MHz analog outputs for microscope control (Galvos, Piezos, Pockels Cells, ...)
- 12 1MHz analog inputs for actuator feedback
- 32 digital inputs/outputs for triggering / clocking
Linear Scanning
For linear Scanning, ScanImage supports two hardware platforms
- vDAQ (recommended)
- NI DAQ hardware (low cost)
vDAQ for linear scanning
See vDAQ Configuration for more information.
NI hardware for linear scanning
| Role | Item(s) | Notes |
|---|---|---|
PMT acquisition, galvo control | PCIe-6374 | For multi-PMT acquisition, a simultaneously sampled NI X-series board is required. |
Optional
| Role | Item(s) | Notes |
|---|---|---|
| Pockels control | PCIe-6321 OR PXIe-6341 | Highly recommended - allows blanking laser during resonant scanner slowdown and galvo scanner flyback |
| Piezo/FastZ control | PCIe-6321 OR PXIe-6341 | Allows for volume imaging applications |
Attachments:
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vDAQ2 breakout render.png (image/png)
vDAQ2 render_1.png (image/png)