Contents

Scanhead Hardware
Acquisition and Control Hardware
Introduction
Resonant Scanning
Linear Scanning
Preamplifier Hardware
Computer Hardware
Piezo Hardware (Optional)
Stage Hardware (Optional)
SLM Hardware (Optional)
Camera Hardware

Scanhead Hardware

ScanImage supports a wide variety of scanning systems including linear (galvo/galvo, GG) and resonant/galvo (RG) systems. Resonant scanning with multiple
regions of interest (MROI) is supported for GG and resonant/galvo/galvo (RGG) systems. Parallel scanning systems are supported to provide simultaneous imaging
and photostimulation.

Supported scanning configurations can be ordered as a preassembled system, or as individual components.

Vendor
	Rapid Multi-Region Scanner system Includes control box and cables Compatible with Sutter MOM, Thorlabs Bergamo and DIY microscopes
	MOM-Resonant microscope system Resonant Scan Box kit Part of Janelia Microscope system design
	Bergamo microscope 2 (BScope 2) systems Bergamo microscope 1 (BScope 1) systems Acerra microscope systems Multiphoton Essentials Kit
	M ultiphoton Resonant System M ultiphoton Imaging System VivoScope System
Cambridge Technology & others	ScanImage Core Scanner Support

Acquisition and Control Hardware

Introduction

ScanImage 2015 and later allows users to configure their system for resonant scanning or linear scanning (=galvo only scanning). A microscope with two laser paths can combine both methods to allow for simultaneous imaging and photostimulation.

The sections below describe the basic acquisition and control hardware requirements for both imaging systems.

Resonant Scanning
Linear Scanning (Galvometric scanning)

Resonant Scanning

ScanImage uses vDAQ for data acquisition and microscope control. vDAQ is an all-in-one data acquisition board that controls the most advanced laser scanning microscopes.

vDAQ PCIe

The 2U rackmountable vDAQ breakout shows the main functionality of the data acquisition system:

4 fast analog inputs for acquiring PMT data
12 2MHz analog outputs for microscope control (Galvos, Piezos, Pockels Cells, ...)
12 1MHz analog inputs for actuator feedback
32 digital inputs/outputs for triggering / clocking

Linear Scanning

For linear Scanning, ScanImage supports two hardware platforms

vDAQ (recommended)
NI DAQ hardware (low cost)

vDAQ for linear scanning

See vDAQ Configuration for more information.

NI hardware for linear scanning

Role	Item(s)	Notes
PMT acquisition, galvo control	PCIe-6374 OR PXIe-6356	For multi-PMT acquisition, a simultaneously sampled NI X-series board is required.

Optional

Role	Item(s)	Notes
Pockels control	PCIe-6321 OR PXIe-6341	Highly recommended - allows blanking laser during resonant scanner slowdown and galvo scanner flyback
Piezo/FastZ control	PCIe-6321 OR PXIe-6341	Allows for volume imaging applications

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Users upgrading from ScanImage 3.8 can reuse their existing PCI-6110/6132/6133 digitizers, but will need to purchase at least one additional X-series board (e.g. PCIe-6321). Please contact us at support@vidriotech.com to learn about upgrade options from ScanImage 3.x

Configuration Guide

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Please see Configuring LinScan (Galvo Galvo scanning) page for additional details.

Preamplifier Hardware

Photomultiplier tubes require a transimpedance amplifier for recording by ScanImage.

The optimal bandwidth depends on the sample rate and shortest pixel durations.

For resonant scanning a bandwidth of at least 30 Mhz is recommended.

The Femto DHCPA-100 is a commercial off-the-shelf option suited for ScanImage 5 and later, at its 80MHz setting.

Thorlabs also makes a usable transimpedance amplifier. Please contact them directly for a part number and quote.

Computer Hardware

	Minimum Recommended
CPU	64-bit processor with 4 cores, ≥ 3GHz Examples: Current recommended processors are the i7-9700K. An i5 versions of this processors will work but provides lower performance. Processors/systems with at least 6 cores may be valuable for future ScanImage or user-specific requirements combining TPLSM imaging with other simultaneous processing/acquisition Icon Matlab is single threaded in its base configuration, so that a high CPU clock rate is desirable. The fastest single thread processor is currently the i7-9700K. Xeon processors are poor choices for running ScanImage.
RAM	16 GB RAM Additional RAM may be valuable for future ScanImage or user-specific requirements, e.g. online image analysis done in User Functions
PCIe slots	(usually) PCIe x16 for a graphics card. (required) PCIe x1 for MXI controller connecting to the PXI chassis If PCIe based DAQ's (e.g. PCIe-6341) are used for Beams and Fast Z Concepts features, then additional PCIe slots will be required. Only the PCIe x1 slot for the MXI controller card is strictly required when using a PXIe chassis. Additional PCIe slots may be valuable for future ScanImage or other user-specific requirements
Hard Drives	A separate drive or partition for OS and data logging/storage is recommended, to avoid contention. Either a RAID 0 magnetic drive pair or solid-state drive (SSD), whole or partition, is recommended for data logging/storage, to achieve write performance >300Mb/second. Examples: Drive for OS plus separate solid-state drive (SSD) for data logging/storage OR Drive for OS plus two 7200 rpm hard drives in RAID 0 configuration to achieve 300 Mb/sec write speed for data logging M.2 drive for OS provides optimal performance for ScanImage Ultra-fast data storage > 5Gb/sec can be achieved if necessary.
OS	Windows 7 Professional Windows 8.1 Professional Windows 10 Professional

Piezo Hardware (Optional)

Vendor	Controller	Compatible Piezos	Comments
Physik Instrumente	E-665 E-709 (CHG) E-753	PIFOC piezos (e.g. P-721, P-725, P-725.xDD, P-725KHDS)
nPoint	LC.40x	nPFocus objectives	Options from 100um to 1000um of travel
Thorlabs	PFM450
Generic			Any focusing device accepting a -10V..10V analog command signal.

Stage Hardware (Optional)

Vendor	Stage Controllers	SI Free	SI Premium
Sutter	MP-285, MPC-200
Tholabs	MCM3000, MCM5000, MCM6000, Bergamo II Scope, DDSM100, MLJ150, Z812B, Z825B
Scientifica	Patchstar (2), Slice Scope UMS (2), MMTP. MMSP, MMBP, IMTP
Physik Instrumente	E-665, E-709 (CHG), E-753, C-884, C-863
nPoint	LC.400 Series
ASI	MS-2000
Bruker	MAMC
Zaber	X-DMQ12P, X-LRM050A, X-LRQ300, X-VSR40A
Newport*	XPS, ESP301
Galil	DMC 4040
Prior	ProScan III

*Requires MATLAB Instrument Control Toolbox

SLM Hardware (Optional)

Vendor	Controller
Meadowlark Optics	PCIe8 (ODP series)
Meadowlark Optics	PCIe16
Meadowlark Optics	1920x1152 SLM
-	DVI/HDMI controlled SLM

Camera Hardware

Vendor	Type
FLIR/Pointgrey	USB3, GigE
uManager	Variety of cameras

ScanImage 2019 : Supported Microscope Hardware