Introduction
ScanImage 2015 and later allows users to configure their system for resonant scanning or linear scanning (=galvo only scanning). A microscope with two laser paths can combine both methods to allow for simultaneous imaging and photostimulation.
The sections below describe the basic acquisition and control hardware requirements for both imaging systems.
- Resonant Scanning
- Linear Scanning (Galvometric scanning)
Resonant Scanning
ScanImage uses the National Instruments National Intruments FlexRIO platform for the core acquisition and control tasks for resonant two-photon laser scanning microscopy
ScanImage supports a handful of pre-specified FlexRIO system configurations
NI ScanImage Resonant Scanning Bundle
Vidrio Technologies offers National Instruments hardware bundles, to help users create valid configurations. If you also order a ScanImage optimized computer from us at the same time, Vidrio will install ScanImage and create a custom machine data file for your system.
Email us at support@vidriotech.com for a ScanImage hardware bundle quote.
See notes below for further information about the key options
Required
| Role | Item(s) | Performance characteristics |
|---|---|---|
| Chassis/controller | PXIe-1073 or better | |
| Scan Control DAQ | PXIe-6341 | NI PXI-based X Series DAQ |
| Digitizer | One of | NI Digitizer Module
|
| FPGA Module | One of | NI PXI-based FlexRIO Module
For SI5 - there will be no difference in operation. Most users should order the PXIe-7961R. Future versions may utilize the far greater (~10x) processing power of the PXIe-7975R. |
Optional
Additional X Series multifunction DAQ boards for some features:
Linear Scanning
ScanImage uses National Instruments Multifunction DAQ boards for image acquisition and microscope control.
Recommended hardware
| Role | Item(s) | Notes |
|---|---|---|
PMT acquisition, galvo control | NI-6356 | For multi-PMT acquisition, a simultaneously sampled NI X-series board is required. |
Optional
| Role | Item(s) | Notes |
|---|---|---|
| Pockels control | PCIe/PXIe-6341 | Highly recommended - allows blanking laser during resonant scanner slowdown and galvo scanner flyback |
| Piezo/FastZ control | PCIe/PXIe-6341 | Allows for volume imaging applications |
