Integration Scanfield
Definition of an IntegrationField
hSf = scanimage.mroi.scanfield.fields.IntegrationField(); % create an Integration Scanfield hSf.channel = 1; % assign IntegrationField to channel 1 hSf.centerXY = [0.5,0.5]; % set center [x,y] of IntegrationField to center of Reference space hSf.sizeXY = [0.25,0.25]; % set size [x,y] of IntegrationField in Reference space hSf.rotationDegrees = 0; % set rotation of IntegrationField in Reference space hSf.mask = rand(10); % set a mask with weights for underlying pixels hRoi = scanimage.mroi.Roi(); % create empty Roi z = 0; hRoi.add(z,hSf); % add IntegrationField at z = 0 hSI.hIntegrationRoiManager.roiGroup.add(hRoi); % add IntegrationRoi to IntegrationRoiManager
Create IntegrationField by masking entire Imaging Scanfield
hSf_imaging = hSI.hRoiManager.currentRoiGroup.rois(1).scanfields(1); % get the current imaging Scanfield z = hSI.hRoiManager.currentRoiGroup.rois(1).zs(1); % get z of the current imaging Scanfield resXY = hSf_imaging.pixelResolution; % get pixel resolution of current imaging Scanfield mask = zeros(resXY(2),resXY(1)); % create a mask for the scanfield that has the same pixel resolution as the imaging scanfield mask(1:10,1:10) = 1; % set upper left corner to 1 hSf = scanimage.mroi.scanfield.fields.IntegrationField.createFromMask(hSf_imaging,mask); % create IntegrationField from imaging scanfield and mask hSf.channel = 1; % assign IntegrationField to channel 1 hRoi = scanimage.mroi.Roi(); % create empty Roi hRoi.add(z,hSf); % add IntegrationField to Roi hSI.hIntegrationRoiManager.roiGroup.add(hRoi); % add IntegrationRoi to IntegrationRoiManager
Create IntegrationField by masking individual pixels of Imaging Scanfield
hSf_imaging = hSI.hRoiManager.currentRoiGroup.rois(1).scanfields(1); % get the current imaging Scanfield
z = hSI.hRoiManager.currentRoiGroup.rois(1).zs(1); % get z of the current imaging Scanfield
% define mask by assigning a weight to individual pixels in the imaging scanfield
mask = [1,1, 1; ...
1,2, 1; ...
2,1, 1; ...
2,2, 1];
hSf = createFromMask(hSf_imaging,mask); % create IntegrationField from imaging scanfield and mask
hSf.channel = 1; % assign IntegrationField to channel 1
Roi = scanimage.mroi.Roi(); % create empty Roi
hRoi.add(z,hSf); % add IntegrationField to Roi
hSI.hIntegrationRoiManager.roiGroup.add(hRoi); % add IntegrationRoi to IntegrationRoiManager