ScanImage 2018 : How Data and Metadata is Stored

For Z-stepping and 3D multi ROI imaging, how is data and metadata stored?


Data is stored as frames in a TIFF file.

Each frame contains image data for one Z plane, with each subsequent frame being the next Z plane.

Image data for all ROIs imaged on a given Z plane are in each frame.

All metadata is stored in the Tiff file. This includes data defining the shape of all ROIs and the Z-planes that were imaged.