ScanImage is an open-source software application for laser scanning microscopy, electrophysiology, laser scanning photostimulation, and other physiological methods focused on neurobiology.
The software has been previously developed at Cold Spring Harbor Laboratory, Northwestern University , and Janelia Research Campus, with NIH grant support to Gordon Shepherd and Karel Svoboda.
As of 2014, ScanImage is developed and supported by Vidrio Technologies, LLC as both an open-source research resource and as premium software available for sale.
ScanImage is used by over 200 laboratories throughout the world and has been essential to the work described in over 850 publications to date.
Be counted!
If your lab actively uses ScanImage, please let us know about any publications using these tools (if not yet on our list).
License and Citations
ScanImage 5 releases are provided to site members under the ScanImage 5.x License
In addition, we require that those who publish work using ScanImage either:
- cite Pologruto et al
- contact us with news of your publication!
ScanImage
ScanImage is an application for controlling a laser scanning microscope. The application was first released in 2003 and the original version (r3.0) is described in Pologruto et al., Biomedical Engineering Online, 2003.
The latest versions ScanImage 5.1 and ScanImage 2015 are based upon the concept of flexible hardware support including support for multiple scanhead hardware from, Scientifica, Sutter Instruments and ThorLabs.
Data acquisition and control hardware is based upon the powerful National Instruments FlexRIO platform which includes on board FPGA processing for real-time analysis applications.
Please contact support@vidriotech.com for hardware and software license quotes.
View the article ScanImage Free and Premium Comparison to learn more about ScanImage versions.
Version Summary
IconScanImage 2019 was released on June 3rd, 2019 and includes vDAQ support, a revised stack control, Network remote control for ScanImage and advanced SLM features
ScanImage 2018 was released on June 18th, 2018 and includes Photon Counting, 3D Motion Correction, 3P imaging support, and an offline data viewer
ScanImage 2017 was released November 10th, 2017 and adds motion correction in Z, 3D SLM scanning, and much more to ScanImage 2016
ScanImage 2016 was released June 15th, 2016 and adds motion correct, online analysis, arbitrary line scanning and much more to ScanImage 2015
ScanImage 2015 supports combined resonant scanner imaging and galvo scanner photostimulus (and much more)
ScanImage 5.1 supports resonant scanner and galvo scanner imaging
ScanImage 4.1/4.2 supports resonant scanner imaging with specific Thorlabs hardware
ScanImage 3.8 is supports galvo scanner imaging
Premium Releases
Release | Updated | Download | Documentation | README | Summary |
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SI 2019b | 3 December 2019 | Releases have been moved here. | Documentation | README | Live Motor Updates New Stack Controls 3D Shot workflow |
Release | Updated | Download | Documentation | README | Summary |
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SI 2019a | 3 June 2019 | Releases have been moved here. | Documentation | README |
Release | Updated | Download | Documentation | README | Summary |
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SI 2018a | 18 June 2018 | Releases have been moved here. | Documentation | README | Photon Counting using a fast digitizer |
Release | Updated | Download | Documentation | README | Summary |
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SI 2017b | 22 Dec 2017 | The license could not be verified: License Certificate has expired! The license could not be verified: License Certificate has expired!
| Documentation | Z Motion Correction Improved Galvo Waveforms Eliminated FastZ Volume Period Adjustment Custom Header Properties Data Overwrite Warning Various fixes and overhauls Maintenance Update: Bug Fixes for PZAdjust, Tile Display, Rolling Stripe Data, FastZ, Pure Analog Devices + More. |
Release | Updated | Download | Documentation | README | Summary |
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SI 2017a | 30 Aug 2017 | The license could not be verified: License Certificate has expired! The license could not be verified: License Certificate has expired!
| Documentation | 3D SLM targeting and imaging 3D pattern generation and alignment Simultaneous imaging and holographic targeting SLM as FastZ or Linear Scanning device Wavefront generation with Zernike modes Bessel-mode scanning Z-Alignment between stage and focusing device SLM LUT calibration Triggered SLM photostimulation |
Release | Updated | Download | Documentation | README | Summary |
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SI 2016b | 21 March 2017 | The license could not be verified: License Certificate has expired! The license could not be verified: License Certificate has expired!
| Documentation | SLM & Galvo/Galvo targeted Photostimulation, |
Release | Updated | Download | Documentation | README | Summary |
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SI 2015b | 10 March 2016 | The license could not be verified: License Certificate has expired! The license could not be verified: License Certificate has expired!
| Documentation | README | SI5 resonant scanning PLUS: targeted photostimulation; parallel scanners; |
Public Releases
All public release of ScanImage (5.x, 4.x, etc.) have been moved here.
If you already have an account with us, your account will be automatically imported to the new website after logging in using your Confluence credentials.
How to Install
Prerequisites
ScanImage is written primarily in Matlab and uses National Instruments data acquisition (DAQ) hardware for its core functionality.
The Microsoft Windows operating system, Matlab software, and National Instruments DAQ driver software must be installed prior to using ScanImage.
ScanImage Version | OS Version(s) | Matlab Version(s) | NI Driver Versions | |
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2019 / 5.6 | Win7/64-bit OR Win10/64-bit | 2015a up to 2019a (all 64-bit) | DAQmx 9.8 through DAQmx 19.0 FlexRIO 15.5 or later | Install Documentation |
2018 / 5.4 - 5.5 | Win7/64-bit OR Win10/64-bit | 2015a up to 2018a (all 64-bit) | DAQmx 9.8 through DAQmx 18.6 FlexRIO 15.5 or later | Install Documentation |
2017 / 5.3 | Win7/64-bit OR Win10/64-bit | 2015a OR 2015b OR 2016a (all 64-bit) | FlexRIO 15.5 or later | Install Documentation |
2016 / 5.2 | Win7/64-bit OR Win10/64-bit | 2015a OR 2015b OR 2016a (all 64-bit) | FlexRIO 15.5 or later | Install Documentation |
2015 | Win7/64-bit OR Win10/64-bit | 2013b (64-bit) OR 2015a* (64-bit) | Install Documentation | |
5.1 | Win7/64-bit OR Win10/64-bit | 2013b (64-bit) OR 2015a* (64-bit) | Install Documentation | |
5 | Win7/64-bit | 2013b (64-bit) OR 2014a (64-bit) | Install Documentation | |
4.2 | Win7/64-bit | 2011b,2013b | Install Documentation | |
3.8.1 | WinXP/32-bit OR Win7/64-bit | 2011b,2012b,2013b | DAQmx 8.8 OR DAQmx 9.6 OR DAQmx 9.8 |
NO MATLAB TOOLBOXES are required for any currently supported ScanImage version
Multiple Matlab versions can be simultaneously installed, and run, on the same machine/account, without counting as an extra license activation
If using Win7/64-bit and/or using the newer X Series devices from National Instruments, then DAQmx 9.3 or later must be used.
DAQmx version 8.8 compatibility is provided to facilitate upgrading from SI 3.6 or 3.7, which also can run on DAQmx 8.8. With this driver, users can install and switch between ScanImage versions, as desired, during the migration/upgrade.
*Recommended for best performance
ScanImageTiffReader
A library and command line tool for quickly extracting data from Tiff and BigTiff files recorded using ScanImage.
Includes support for C, Python, Matlab and Julia.
Made for Windows x64, Linux and OS X.
Release | Updated | Download | Documentation | Summary |
---|---|---|---|---|
1.3 | 1 November 2018 | See documentation for downloads. |
| |
1.1 | 15 June 2016 |
| Documentation | Initial release. |
Use support@vidriotech.com to:
- submit bug/issue reports
- submit enhancement/feature suggestions
- submit other questions or feedback
Use support.vidriotech.com (login required) to:
- check on the status of tickets you have created
- see your support history
The license could not be verified: License Certificate has expired!