One way to do this is to completely decouple the shutter from the acquisition.
This can be achieved by changing your Machine Data File (MDF):
In the ResScan section, set:
shutterIDs = ;
Now, the shutter(s) don't open anymore when you start scanning.
To open the shutter(s) now, you can use the command line:
- hSI.hShutters.shuttersTransition(1,true); % to open the first shutter
- hSI.hShutters.shuttersTransition(1,false); % to close the first shutter
- hSI.hShutters.shuttersTransition(,false); % to close all shutters if more than one is present in the system
You can write a User Function that uses these commands to automatically open/close the shutters at any point in your experiment.
Useful ScanImage Links: